Numeric Nutrient Criteria Implementation Workshop

2014-6-23 · 16 years working with the Department of Energy and Fluor Daniel, a construction contractor, designing and then implementing clean‐up techniques on a former ... Paul Steinbrecher is the Director of Permitting & Regulatory Conformance at JEA where his primary responsibility is …

Reactive gliosis of astrocytes and Müller glial cells in ...

2011-6-1 · Retinas were incubated with anti-rabbit antibody conjugated with Alexa Fluor 568 (Molecular Probes) for GFAP, and anti-rat antibody conjugated with Alexa Fluor 488 (Molecular Probes) for ER-TR7 in 10% FBS and 10% NGS in PBS for 2.5 h at room temperature. The flat mounted retinas were photographed with a confocal laser scanning microscope system ...

Combinatorial approach to the development of fluorescent ...

2000-12-2 · The development of molecular sensors is an important goal of broad consequence, ranging from environmental monitoring to the study of intracellular processes. 1 For instance, fluorescent probes for measuring intracellular concentration of calcium ions have afforded conceptually new tools for cellular biology. 2 Fluorescence has frequently been used as a readout output due to its high sensitivity.

Technical DaTa SheeT

2021-2-23 · fluor 1 black U bottom plates (Thermo 7005) were coated with 50µL of antigen (5µg/mL) in PBS containing 0.27 mM EDTA and overnight at 4°C. The wells were washed three times with PBS containing 0.27 mM EDTA. After washing, the plates were blocked with 3% BSA in PBS for 45 min at room temperature. 100µL aliquots of a serial dilu-

Von Bauxit zum Aluminium

2019-9-6 · 18.06.2010 7 Von Christine Vogel und Marcel Weiland Allgemeine Eigenschaften vom Aluminium •Geringe Dichte: 2,6- 2,8g/cm3 (ein drittel der Dichte von Strahl) •Gute chemische, Witterungs- und Seewasserbeständigkeit

Executive News: Hatch Names NYC Subway Veteran To Boost …

2017-7-23 · Fluor announces its second-quarter results on Aug. 3. ... Paul Steinbrecher, director of permitting and regulatory conformance for JEA, the largest community-owned utility company in Florida; ...

Immune complexes containing malondialdehyde (MDA) LDL ...

2018-2-1 · Briefly, after treatment with medium alone as control or with oxLDL, MDA-LDL, oxLDL IC or MDA-LDL IC, macrophages were washed with cold 0.01 M PBS and Annexin-binding buffer, and incubated for 15 min with Annexin V-Alexa Fluor 488 (50 μg/mL) and Hoechst 33,342 (5 μg/mL) to detect nuclei. Macrophages were then washed with Annexin-binding ...

Deletion of bone marrow myeloperoxidase attenuates …

2021-1-1 · Goat anti-rabbit IgG (Alexa Fluor 555, Jackson Immunoresearch Laboratories, PA) and anti-rat IgG (Alexa Fluor 488) (Cedarlane, NC) were used as secondary antibodies. Dual immunofluorescence labeling was simultaneously scanned by an Olympus FV500 confocal laser-scanning microscope, equipped with a complete integrated image analysis software ...

Alternative splicing of clathrin heavy chain contributes ...

Alexa Fluor 488 and Alexa Fluor 568 were sequentially excited using lasers with wavelengths of 488 and 561 nm, respectively. Images were acquired at super resolution using a LiveSR module (Gataca Systems) and Metamorph 7.4 software. ... Steinbrecher, and . A.S. Ulrich. 2014.

Ceramide and palmitic acid inhibit macrophage-mediated ...

2020-3-28 · Accumulating evidence indicates that ceramide (Cer) and palmitic acid (PA) possess the ability to modulate switching of macrophage phenotypes and possess anti-tumorigenic effects; however, the underlying molecular mechanisms are largely unknown. …

Visualization of self-delivering hydrophobically modified ...

To this end, we used sd-rxRNA-Cy3 in conjunction with Alexa Fluor 647-labeled EGF (EGF-Alexa647) and Alexa Fluor 488-labeled Tf (Tf-Alexa488). COS-7 cells were simultaneously treated with 250 nM sd-rxRNA-Cy3, 2 ng/μl EGF-Alexa647 and 25 ng/μl Tf-Alexa488. After 10 min, the cells …

Recognition of lipoproteins by scavenger receptor class A ...

2021-8-1 · The scavenger receptor (SR) was first identified in late 1970s because of its binding with the modified low-density lipoproteins (LDLs) ().Over the past decades, a large number of SRs have been found with wide tissue distributions and play important roles in maintaining homeostasis and host defense by recognizing both self- and non–self-ligands (2, 3, 4), including proteins, carbohydrates (5 ...

Novel role for SLPI in MOG-induced EAE revealed by spinal ...

2008-5-26 · Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte protein (MOG) in Dark Agouti (DA) rats is a chronic demyelinating animal model of multiple sclerosis (MS). To identify new candidate molecules involved in the evolution or repair of EAE-lesions we used Affymetrix oligonucleotide microarrays to compare the spinal cord transcriptome at the peak of EAE ...

Direct extracellular interaction between the early ...

2007-5-7 · For analysis of the binding of ESAT-6 to the cell surface, treated cells were incubated with anti-histidine, were stained with Alexa Fluor–conjugated secondary antibody and were analyzed on a ...

Role of Adiponectin‐Notch pathway in cognitive dysfunction ...

2021-5-30 · The used secondary antibodies used including: Alexa Fluor 546, Goat anti-Rabbit (1:800, A-11035, Life Technologies); IRDye ® 800CW Donkey anti-Goat IgG (1:10,000, 925-32214, LI-COR Biosciences); IRDye ® 800CW Donkey anti-Rabbit IgG (1:10,000, 925-32213, LI-COR Biosciences); IRDye ® 680RD Donkey anti-Mouse IgG (1:10,000, 925-68072, LI-COR ...

Single-cell analysis reveals effective siRNA delivery in ...

2021-4-30 · Next, the sections were incubated with goat anti-rabbit Alexa Fluor 488 secondary antibody diluted in 1% bovine serum albumin (1:250; A31556, Invitrogen) for 1 hour at room temperature. To stain the cell nucleus, samples were incubated with DAPI diluted in PBS (1:1000; 62248, Invitrogen) for 10 min after washing.

Guanylin and uroguanylin are produced by mouse intestinal ...

2016-5-31 · Guanylin (GN) and uroguanylin (UGN), through activation of guanylyl cyclase C (GCC), serve to control intestinal fluid homeostasis. Both peptides are produced in the intestinal epithelium, but their cellular origin has not been fully charted. Using quantitative …

Fatty Acid-binding Proteins (FABPs) Are Intracellular ...

2015-4-3 · Incubation with Alexa Fluor 488 and Alexa Fluor 555-conjugated secondary antibodies at 1:1000 was performed at room temperature for 1 h, followed by washing with PBS and mounting using Fluoromount-G with DAPI (Southern Biotech). 4–8 brain sections were imaged per biological replicate. Statistics

5-Benzylidene-hydantoin is a new scaffold for SIRT ...

2016-3-31 · Reaction was stopped by the addition of Fluor de Lys developer® II (KI176, Enzo) supplemented with nicotinamide (2 mM final concentration, Sigma Aldrich). Fluorescence readings were obtained using FLx800 reader (BioTek) with an excitation wavelength of 360 nm and an emission of 460 nm. Sirtinol was used as a positive control.

Ubiquitylation of MLKL at lysine 219 positively regulates ...

2021-6-7 · The following antibodies were used for the spleen analysis: Fixable Viability Dye eFluor 780 (eBioscience 65-0865-14, 1:1000), Alexa Fluor 700 anti-mouse CD45 clone 30-F11 (BioLegend 103128, 1:200 ...

Binding of single nucleotides to H+-ATP synthases observed ...

2004-6-1 · The diffusion of free ATP-Alexa Fluor 647 and F 1-bound ATP-Alexa Fluor 647 was investigated with FCS. Fig. 2d shows the autocorrelation functions G ( τ c ) [17] . By fitting these data, we obtained translational diffusion correlation times of 0.49 ms for free ATP-Alexa Fluor 647 and 2.3 ms for F 1 -bound ATP-Alexa Fluor …

Uroguanylin increases Ca2+ concentration in astrocytes via ...

Alexa fluor 488 was excited by 488 nm argon laser line, and the fluorescent emission was collected from 505-530 nm. Cy5 labeling glial fibrillary acidic protein (GFAP) or specific neuronal marker neuronal nuclei marker (NeuN) was excited by 633 nm HeNe laser line, and the fluorescent signal was collected from 650-680 nm.

Intravital Imaging of Neocortical Heterotopia Reveals ...

Following primary antibody incubation, slices were washed in PBS and then incubated with Alexa Fluor dye-conjugated secondary antibodies of the appropriate host species at 1:600 dilution in 0.1% Triton X-100 and 5% NGS in PBS for 1–2 days at 4 °C.

Frontiers | Lipidomics Revealed Alteration of Sphingolipid ...

2021-3-12 · Seven μm frozen sections of heart were fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 30 min, blocked with 1% BSA for 30 min, and incubated with primary antibodies at 4°C overnight, then with Alexa fluor-conjugated secondary antibodies. Fluoroshield mounting medium with DAPI was used to cover slides.

# Fluor • Praxislexikon

2021-4-14 · An welchem Wochentag wurde ich geboren? Tag (1-31) Monat (1-12) Jahr (z. B. 1960) Geburtstag: JavaScript von kostenlose-javascripts

Antisense correction of SMN2 splicing in the CNS rescues ...

Steinbrecher U, Bennett CF; Butler M, Crooke RM, Graham MJ, Lemonidis KM, Lougheed M, Murray SF, Witchell D, Steinbrecher U, Bennett CF. 2000. Phosphorothioate oligodeoxynucleotides distribute similarly in class A scavenger receptor knockout and wild-type mice. J Pharmacol Exp Ther 292: 489 – 496.

Minimally Oxidized LDL Offsets the Apoptotic Effects of ...

2006-2-16 · Briefly, at the end of FC-loading, cells were gently washed twice with PBS, and then incubated in 100 μL annexin-binding buffer (25 mmol/L HEPES, 140 mmol/L NaCl, 1 mmol/L EDTA, pH 7.4, 0.1% bovine serum albumin) containing 5 μL of Alexa Fluor 488 annexin V, and 1 μL of 100 μg/mL propidium iodide (PI) for 15 minutes at room temperature.

Fruit fracture biomechanics and the release of Lepidium ...

2017-11-30 · Sperber, K., Steinbrecher, T., Graeber, K. et al. Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi. Nat Commun 8, 1868 (2017). https ...

Oligo-guanosine nucleotide induces neuropilin-1 ...

2010-10-21 · Cells were then stained with Alexa Fluor 488– or 546–conjugated streptavidin (Invitrogen), mouse anti–human NRP1 mAb, goat anti–human SREC-I Ab, rabbit anti–human lysosome-associated membrane protein-2 (LAMP2) Ab (Abcam), or rabbit anti-phospho VEGFR-2 (Y1175) mAb (19A10; Cell Signaling Technology) in PBS with 3% BSA and 3% FBS at 4 ...

Implication of matrix metalloproteinases 2 and 9 in ...

2016-8-1 · MMP-2 and -9 play critical roles in the regulation of cell chemotaxis, .The implication of these gelatinases in the stimulation of macrophage migration by C1P was tested using (2R)-2-[(4-Biphenylylsulfonyl)amino]-3-phenylpropionic acid, which selectively inhibits the activity of both MMP-2 and -9, as well as specific siRNA to silence the genes encoding these MMPs.

Single-cell analysis reveals effective siRNA delivery in ...

RNA-based therapies offer unique advantages for treating brain tumors. However, tumor penetrance and uptake are hampered by RNA therapeutic size, charge, and need to be "packaged" in large carriers to improve bioavailability. Here, we have examined delivery of siRNA, packaged in 50-nm cationic lipid-polymer hybrid nanoparticles (LPHs:siRNA), combined with microbubble-enhanced focused ...

Rabbit anti-Human PDE2A antibody (ABIN2747086)

Alexa Fluor 488 1; Alexa Fluor 555 ... Sprenger, Perera, Steinbrecher, Lehnart, Maier, Hasenfuss, Nikolaev: "In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in …

Immissions-, Depositions-, und Dieselmotoremissions ...

2017-10-26 · Borrmann und Hr. Aburous; Hr. Steinbrecher, Hr. Thele und Hr. Rietdorf für die Konstruktion der Probenahmegeräten. Ganz herzlich danke ich Pfr. Kim und meine Brüder und Schwester von Banseok Gemeinde in Berlin und Pfr. Shin, Jongmin Kim, und Jongwoon Hwang in Saarbrücken. Zum Schluss möchte ich mich bei meiner Familie - Jungyoun, Damin ...

Investigation of the biophysical properties of a ...

2016-10-1 · At TopFluor-C1P concentrations above 10% we observed a self-quenching event, which is typical of Top-Fluor labeled lipids (Kay et al., 2012). This might be explained by the observation that at increasing concentrations of C1P, there is a phase change and formation of non-lamellar structure ( …

A Dystroglycan Mutation Associated with Limb-Girdle ...

2018-1-21 · Beltran-Valero de Bernabe D, Currier S, Steinbrecher A, et al. Mutations in the O-mannosyltransferase gene POMT1 give rise to the severe neuronal migration disorder Walker-Warburg syndrome. Am J ...

Probing the Charge-Transfer Dynamics in DNA at the Single ...

2011-9-12 · Photoinduced charge-transfer fluorescence quenching of a fluorescent dye produces the nonemissive charge-separated state, and subsequent charge recombination makes the reaction reversible. While the information available from the photoinduced charge-transfer process provides the basis for monitoring the microenvironment around the fluorescent dyes and such monitoring is particularly important ...

The lytic polysaccharide monooxygenase CbpD promotes ...

2021-2-23 · The recently discovered lytic polysaccharide monooxygenases (LPMOs), which cleave polysaccharides by oxidation, have been associated with bacterial virulence, but supporting functional data is …

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